rabbit polyclonal antibody against pampk  (Cell Signaling Technology Inc)

Journal: International Journal of Molecular Sciences

Article Title: Adiponectin Exerts Peripheral Inhibitory Effects on the Mouse Gastric Smooth Muscle through the AMPK Pathway

doi: 10.3390/ijms21249617

Figure Lengend Snippet: Involvement of AMPK signaling in ADPN action. ( A ) Effect of ADPN on AMPK signaling pathway in mouse gastric fundus assayed by Western blotting: representative bands from a typical experiment. ( B ) quantitative analysis. Columns are means ± SEM. Significance of differences (Student’s t -test for two independent samples): * p < 0.05 vs. controls (CTRL). ( C ) Representative photomicrographs of gastric tissue from control and ADPN-treated mice showing double immunofluorescence labeling. ( A1–A4 ) co-localization of phosphoAMPK (pAMPK) and GFAP in control mice: ( A1 ) pAMPK signal (green channel); ( A2 ) GFAP signal (red channel); ( A3 ) DAPI; ( A4 ) merged images. ( B1–B4 ) co-localization of pAMPK and GFAP in ADPN-treated mice: B1 pAMPK signal (green channel); ( B2 ) GFAP signal (red channel); ( B3 ) DAPI; ( B4 ) merged images. Scale bar: 10 µm.

Article Snippet: To evaluate AMPK and pAMPK expression, gastric tissue sections from control and ADPN-treated mice were processed as above and incubated overnight at 4 °C with rabbit polyclonal anti-pAMPK antibody (1:50, Cell signaling) or rabbit polyclonal anti-AMPK (1:50; Cell Signaling) followed by goat anti-rabbit Alexa Fluor 488-conjugated IgG (1:175 Invitrogen) for 2 h at RT.

Techniques: Western Blot, Control, Immunofluorescence, Labeling